Louis Pasteur (1822 – 1895)

Louis Pastuer was a French chemist and microbiologist born in Dole. He is remembered for his remarkable breakthroughs in the cause and prevention of diseases. His discoveries reduced mortality from puerperal fever, and he created the first vaccine for rabies and anthrax. His experiments supported the germ theory of disease. He is best known to the general public for inventing a method to stop milk and wine from causing sickness, a process that came to be called pasteurization. He is regarded as one of the three main founders of microbiology, together with Ferdinand Cohn and Robert Koch.

Message of the Chair

Dr.C.Revathy MD,

Professor & Head of the Department

Message of the Chair
Established in 1967 under the broad umbrella of Pathology & Bacteriology, this department evolved into an independent Department of Microbiology under the leadership of Dr.Ramajeyam. The department presently serves a nodal point for microbiology diagnosis, research and teaching for the southern districts of the State of Tamilnadu. This department has grown in tandem with the medical needs of our patients and the society around. A Voluntary Counseling & Testing Centre for HIV was established in 1999, which was later elevated to a Regional Reference Laboratory under NACO in 2000 and a Integrated Counseling & Testing Centre in 2002. Molecular Diagnostics Lab was established in 2010.

Succession of Heads of Department
Dr.Ramajeyam  MD DCP1977-1984
Dr.Ganapathy Sundaram MD 1984-1996
Dr.Vasantha Babu MD 1996-2001
Dr.V.Gomathy MD 2001-2003
Dr.Indra Priyadharshini MD 2003-2008
Dr.N.Palaniappan MD20082014
Dr. C.Revathy 2014On Date

Diagnostic Program


Samples are received at the Common Collection Centre of the Central Diagnostic Laboratory for OPD patients. Samples are collected at the bedside for patients indoor.

Samples of Blood, Urine, Stool, Body fluids – Peritoneal Fluid, Pleural Fluid, Cerebrospinal Fluid, Sputum, Gastric Lavage, Bronchiolo-Alveolar Lavage, Pus, Discharge from Drain Tubes, Tracheostomy Site, Wound Swabs, Indwelling Catheter tips, Vascular grafts, Seminal fluid, Throat swabs, Rectal swabs, Vaginal swabs, Ear swabs, Nasal swabs, Swabs from Organ Transplant Patients, Blood Bags, Postmortem Specimens, Theatre Floor & Station Swabs. The Program includes labs that test for Bacteria: with all simple & compounded stains - Gram stain, Acid Fast stains for TB & Lepra, Modified acid fast stain, Fontana’s stain (Leptospira), Wet Mounts: for Stool Samples for ova and cyst, Hanging Drop method for motility study in Vibrio Cholerae, Dark Ground Microscopy For Leptospira, Culture: Aerobic and Anaerobic cultures with Antibiotic Susceptibility Tests: Antibiotic sensitivity tests are done for all the bacteriology positive isolates.

ASO Titres, Widal Serology for Typhoid, Brucella, RA Factor, Chickungunya, Swine Flu, Bird Flu, Dengue, HIV, HBV, HCV, HEV, & HAV.

Fungus Studies with Lacto-phenol-cotton Blue Stain, India-Ink , Nigrosin stain, Potassium hydroxide mount, sliver stains & Fungus Cultures.

LPCB mount for stool ova and cyst. & Microscopy with Special stains.


  • Infection Control in Tirunelveli Medical College Hospital.
  • Development of Antibiotic Policy for Tirunelveli Medical
  • College Hospital.
  • In-house monitoring of the safety of water in the TVMC & TVMCH campus.
  • Monitoring of Operation Theatres in TVMC, and other Government and Corporate Medical Institutes in Tirunelveli District.
  • Monitoring of Biomedical Waste Management and Training of Stakeholders
  • Maintenance of Mother cultures for research and diagnostic activities.
Year 2007 2008 2009 2010 2011 2012
CLINICAL MICROBIOLOGY 22814 25710 25840 26435 32038 30042
BACTERIOLOGY 38496 44130 49639 49924 49552 64761
MYCOLOGY 293 204 288 292 402 520
VIROLOGY & SEROLOGY 15334 17286 18432 18849 18858 24846
PARASITOLOGY 157 120 172 132 185 124


Academic Program

The department trains graduate medical students during the 3rd to 5th semesters. The curriculum, affiliated to the Tamilnadu Dr.M.G.R.Medical University integrates theoretical and practical training to the 150 students every year. The students are also encouraged to undertake Short term projects under ICMR during the course period. The course includes a planned yearly calendar of didactic lectures, seminars, symposia, integrated sessions and clinical interaction sessions. The department also encourages the students to undertake community studies in microbiology.

The department has 15 residency posts with 5 fresh intakes a year. The residents are taken through a program as per the Tamilnadu Dr.M.G.R.Medical University regulations and integrate theoretical, clinical and practical training spread over three years. The students are also encouraged to undertake Short term research projects and make a detailed research thesis to be completed and presented before the end of the three years. The course includes a planned yearly calendar of three monthly residency posts with didactic lectures, seminars, symposia, integrated sessions, clinical interaction sessions, journal clubs and case discussions. The department also encourages the students to undertake community studies in microbiology.

Eligibility: M.B.B.S. Degree from any Indian university recognized by the Medical Council of India & and obtained permanent registration from any of the State Medical Councils.

Course duration: 3 years.

Selection procedure: Selection for two/three of the five seats of residents will through the Tamilnadu Post Graduate Entrance Examination and the remaining three/two seats through the All India Post Graduate Entrance Examination conducted by DGHS, New-Delhi.

Academic Curriculum: The objective of the program is to: (1) Establish good clinical microbiological services in a hospital and in the community in the fields of bacteriology, virology, parasitology, immunology and mycology. (2) Plan, execute and evaluate teaching assignments in Medical Microbiology.(3) Plan, execute, analyze and present the research work in Medical Microbiology.(4) Conduct such clinical and experimental research, as would have a significant bearing on human health and patient care.(5) Encourage interaction with the allied departments by rendering services in advanced laboratory investigations and relevant expert opinion.(6) Encourage the student to participate is various workshops / seminars / journal clubs / demonstration in the allied departments, to acquire various skills for collaborative research.(7) Uphold the prestige of the discipline amongst the fraternity of doctors.(8) Undergo specialization in any of the sub-specialties

Research Program


  1. Analysis of malaria diagnostic tools – Microscopy and Rapid Antigen detection
  2. Comparative study of IgM ELISA and WIDAL in the diagnosis of Typhoid fever
  3. Detection of baseline titer of antibody to HBsAg in non- vaccinated health care workers
  4. Comparison of MAT and Immunochromatography in the diagnosis of leptospirosis
  5. Diagnostic value of anticyclic citrullinated peptide antibodies compared with Rheumatoid factor in patients with rheumatoid arthritis.
  6. Seropositivity of anti- rubella antibodies among health care workers.
  7. Comparative study of Dot enzyme immunoassay and Widal test in the diagnosis of typhoid fever.
  8. Serological profile of HSV – 2 in STD patients: Evaluation of diagnostic utility of HSV-2 IgM and IgG detection. (ICMR,NewDelhi)
  9. Microbiologic profile of chronic suppurative otitis media in a tertiary care hospital.
  10. Detection of MBL producing Pseudomonas aeruginosa in Tirunelveli medical college hospital.
  11. Clinico-mycological profile of Dermatophytes in Tirunelveli medical college hospital.
  12. Asymptomatic bacteriuria in healthy individuals in Tirunelveli district
  13. Comparison of stool antigen with PCR in gastric biopsy material of Helicobacter pylori
  14. Prevalence of Syphilis in normal population and high risk population.
  15. Evaluation of conventional methods and molecular methods for the detection of Carbapenamase among clinical isolates of Enterobacteriaceae.
  16. Prevalence of ESBL among Enterobacteriaceae in urinary specimens in Tirunelveli Medical College Hospital
  17. Clostridium difficile infection in Tirunelveli Medical College Hospital.
  18. Prevalence of Dengue IgG in normal population
  19. Detection of CMV by ELISA and PCR in immunocompromised patients.
  20. A rapid latex agglutination test for qualitative and semi qualitative detection of Toxoplasma gondii antibodies in different patient categories. (ICMR,NewDelhi)
  21. Occurrence of ESBL and MBL in clinical isolate of Pseudomonas Aeruginosa – an emerging threat to clinical therapeutics. (ICMR,NewDelhi)
  22. UTI in elderly – A clinical and Microbiological study. (ICMR,NewDelhi)
  23. The significance of CSF Adenosine Deaminase and CRT estimation in meningitis in adults (ICMR, NewDelhi).
  24. Comparison of diagnostic methods in bacterial vaginosis in a tertiary care hospital.
  25. Prevalence of allergic fungal sinusitis among candidates for functional endoscopic sinus surgery. (ICMR,NewDelhi)
  26. Comparison of Immunochromatography with RTPCR for detection of Rotavirus in fecal samples.
  27. Clinical Chikungunya cases – A follow up serology after 1-2 years.
  28. Evaluation of Chromagar and PCR for detection of MRSA from clinical isolate.
  29. Comparison of Rapid Immunochromatography test with ELISA for Anti HCV antibody detection in patients on hemodialysis.
  30. Seroprevalence of Brucellosis among animal handlers.
  31. Asymptomatic candiduria and species identification in Diabetic patients.
  32. Association of ANA in pregnant women with bad obstetric history.
  33. Baseline widal titer among healthy volunteer.
  34. Serodiagnosis of Dengue in pediatric patient – Importance of NS1 Antigen.
  35. Prevalence of Streptococcal throat carriage in children.
  36. Bacteriological profile and antibiogram of chronic obstructive pulmonary disease cases – A prospective study. (ICMR,NewDelhi)
  37. Use of Adenosine deaminase as a rapid diagnostic tool for TB in pleural effusion. (ICMR,NewDelhi)
  38. Phenotypic differentiation of BORSA from MRSA : Comparison of susceptibility testing methods and MRSA LAT. (ICMR,NewDelhi)
  39. An investigation of microbiological contamination of ultrasound probes: Evaluation of cleaning methods to reduce it. (ICMR,NewDelhi)
  40. Study of prevalence of clindamycin resistance among Staphylococcus.
  41. Prevalence of HCV infection among haemodialysis patients.
  42. Efficacy of Hepatitis B vaccines in health care workers.